Dictyostelium discoideum (ameoba) phagocytosing budded yeast (S. cerevisiae). The amoeba is expressing mRFP-LimEdelta (labels actin filaments, red) and PHcrac-GFP (labels membranes containing phospha...

Zebrafish embryo at the 5-somite stage The cross-sectional view is in the ventral portion of the neural keel. In the lower portion of the image, somites detach from ventral mesoderm. Oriented division...

Time series of cooled CCD fluorescence micrographs of live amoeba syringe-loaded with Rho-N58C-PII. See publication below for details. Live cells were observed with a Zeiss Axiovert 135 fluorescence m...

The single cell amoeboid phase of the life cycle of the slime mold Dictyostelium discoidium moves over the substrate by generating cytoplasmic outgrowths known as pseudopodia. In this time lapse movie...

Severing of budded yeast. Amoebae (D. discoideum) were plated with yeast (S. cerevisiae) in a glass bottom chamber and viewd by confocal microscopy. The amoebae were expressing mRFP-LimEdelta (red) to...

A live amoeba treated with the membrane-binding vital dye FM4-64 which stains the plasma membrane, contractile vacuole and other organelles. Large membrane-bounded organelles are excluded from the bro...

To characterize and validate the gluteraldehyde fixation method, a live amoeba syringe-loaded with Rhodamine-N58C-profilin-II was monitored in a flow cell as the fixative was introduced. This time se...

Starved wild-type Dictyostelium cells migrate toward the cathode (left). A direct-current electric field of 10 Vpcm was applied and the cells were observed for 20 min at 5-sec intervals. (Scale bar, ...

Phagosome neutralization visualized using FITC-yeast. Amoebae of Dictyostelium discoideum were fed living budding yeast (S. cerevisiae strain TH2-1B) that had been labeled with fluorescein isothiocyan...

A movie of phagosome neutralization visualized using FITC-yeast. Amoebae of Dictyostelium discoideum were fed living budding yeast (S. cerevisiae strain TH2-1B) that had been labeled with fluorescein ...